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. 2020 Apr 7;21:e00160. doi: 10.1016/j.plabm.2020.e00160

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© 2020 Published by Elsevier B.V.

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Fig. 4 — Method comparison between the in-house vitamin B6 assay with/without NIST calibration and a reference laboratory method (n = 26). Left panel: A. Comparison of the in-house HPLC method with a reference lab HPLC method showed equivariant measurement of PLP in plasma specimens using absorbance assigned calibrator values. B. Bias was observed between the in-house HPLC method and the reference lab method after the in-house calibrators were calibrated against the NIST standard reference material 3950. The black dashed line in panel A and B indicate the 1:1 line.