Table 2.
Synaptic branch loss in fixed muscles.
| Number of branch lost | sternomastoid |
soleus |
EDL |
|||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 4 mo |
6 mo |
9 mo |
4 mo |
6 mo |
9 mo |
4 mo |
6 mo |
9 mo |
||||||||||
| SD | HFD∗∗ | SD | HFD✝ | SD | HFD✝ | SD | HFD a | SD | HFD∗∗ | SD | HFD✝ | SD | HFD a | SD | HFD∗ | SD | HFD✝ | |
| 0–1 | 132 (90%) | 147 (72%) | 96 (65%) | 81 (47%) | 72 (65%) | 51 (32%) | 38 (100%) | 50 (96%) | 40 (93%) | 49 (68%) | 159 (90%) | 78 (56%) | 40 (98%) | 45 (92%) | 28 (93%) | 52 (76%) | 105 (91%) | 81 (58%) |
| 2–5 | 14 (10%) | 51 (25%) | 51 (35%) | 69 (40%) | 35 (31%) | 75 (46%) | 0 | 2 (4%) | 3 (7%) | 23 (32%) | 17 (10%) | 54 (38%) | 1 (2%) | 4 (8%) | 2 (7%) | 16 (24%) | 10 (9%) | 52 (37%) |
| >6 | 0 | 7 (3%) | 0 | 23 (13%) | 4 (4%) | 36 (22%) | 0 | 0 | 0 | 0 | 0 | 8 (6%) | 0 | 0 | 0 | 0 | 0 | 6 (4%) |
| Total NMJs | 146 | 205 | 147 | 173 | 111 | 162 | 38 | 52 | 43 | 72 | 176 | 140 | 41 | 49 | 30 | 68 | 115 | 139 |
a Values are not statistically significant; ∗and ∗∗ values indicate significance compared to age-matched Standard Diet (p<0.05 and p<0.001 respectively);† Indicates significance compared to age-matched Standard Diet (p<0.0001). 3–4 mice per diet per age.