Table 4.
Experimental design and the results of the collagen extraction process.
| Assay | x 1 | x 2 | x 3 | [Ac] (%) | t (h) | T (°C) | Yexp (%) | Ycalc (%) |
|---|---|---|---|---|---|---|---|---|
| 1 | −1 | −1 | −1 | 1 | 3 | 50 | 4.4384 | 4.2493 |
| 2 | 1 | −1 | −1 | 3 | 3 | 50 | 5.1980 | 5.1505 |
| 3 | −1 | 1 | −1 | 1 | 7 | 50 | 1.6962 | 1.8785 |
| 4 | 1 | 1 | −1 | 3 | 7 | 50 | 5.1659 | 5.4897 |
| 5 | −1 | −1 | 1 | 1 | 3 | 70 | 8.5713 | 8.3821 |
| 6 | 1 | −1 | 1 | 3 | 3 | 70 | 8.1620 | 8.1145 |
| 7 | −1 | 1 | 1 | 1 | 7 | 70 | 2.8835 | 3.0657 |
| 8 | 1 | 1 | 1 | 3 | 7 | 70 | 6.1218 | 6.4457 |
| 9 | 0 | 0 | 0 | 2 | 5 | 60 | 6.2332 | 6.0421 |
| 10 | 0 | 0 | 0 | 2 | 5 | 60 | 6.2700 | 6.0421 |
| 11 | 0 | 0 | 0 | 2 | 5 | 60 | 5.5907 | 6.0421 |
| 12 | 1.682 | 0 | 0 | 3.682 | 5 | 60 | 7.6714 | 7.4079 |
| 13 | −1.682 | 0 | 0 | 0.318 | 5 | 60 | 4.1287 | 4.2016 |
| 14 | 0 | 1.682 | 0 | 2 | 8.364 | 60 | 2.5070 | 1.9702 |
| 15 | 0 | −1.682 | 0 | 2 | 1.034 | 60 | 5.4152 | 5.7617 |
| 16 | 0 | 0 | 1.682 | 2 | 5 | 76.82 | 6.1483 | 6.0530 |
| 17 | 0 | 0 | −1.682 | 2 | 5 | 43.18 | 7.0203 | 6.9252 |
where: [Ac] = acetic acid concentration, t is time, T = temperature, and Y = extraction yield.