Tau antibodies used in this study and the experimental timeline. (A) Schematic representation of 4R0N tau and distinct epitopes recognized by different tau antibodies. (B) Treatment schedule of tetracycline off (TetOff) induction. On day 0, TetOff induction with 1 ng/mL of tetracycline was initiated. On day 4, ROCK inhibitor (H1152, or Y-27632) treatment was initiated. On day 5, cells were harvested. Abbreviation: ROCK, Rho-associated coiled-coil protein kinase.