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. Author manuscript; available in PMC: 2020 Apr 25.
Published in final edited form as: Cell Rep. 2020 Jan 21;30(3):699–713.e4. doi: 10.1016/j.celrep.2019.12.073

Figure 3. IFN Signaling and Translation Initiation Pathways Regulate RIPK-Dependent Inflammation.

Figure 3.

(A) Schematic of sorting process used to compare RNA sequencing and cytokine array results from LPS- and LZ-stimulated B6, Ifnb−/−, and RIP1 Ki BMDMs

(B and C) RNA (log2 FPKM [fragments per kilobase of transcript per million mapped reads] LZ/LPS) (B) and protein (log2 signal intensity LZ/LPS) (C) for cytokines upregulated and downregulated in LZ compared with LPS treatment at the mRNA level for B6, Ifnb−/−, and RIP1 Ki BMDMs.

(D) Extent of global translation as measured by puromycin incorporation in unstimulated, LPS- and LZ-stimulated B6, Ifnb−/−, and RIP1 Ki BMDMs.

(E) Ingenuity Pathway Analysis of RNA sequencing data depicting top ten upregulated pathways for LZ-stimulated Ifnb−/− macrophages.

Statistical significance was determined using Student’s t test: n.s. (p > 0.05) and ****p < 0.0001.