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. Author manuscript; available in PMC: 2020 Apr 25.
Published in final edited form as: Cell Rep. 2020 Jan 21;30(3):699–713.e4. doi: 10.1016/j.celrep.2019.12.073

Figure 5. 4E-BP Activity Blocks RIPK-Dependent Cytokine Translation.

Figure 5.

(A) TNF-α and CXCL-1 protein levels after indicated stimulations ± 5 IU IFNβ priming overnight in B6, Ifnb−/−, 4E-BP TKO, and RIP1 Ki Ifnb−/− BMDMs.

(B and C) TNF-α and CXCL-1 protein levels after indicated stimulations ± treatment with 4EGi-1 (B) or Torin-2 (C) in B6, 4E-BP TKO, and RIP1 Ki Ifnb−/− BMDMs.

(D) Cell death as measured by propidium iodide incorporation over 6 h in B6, Ifnb−/−, 4E-BP TKO, and RIP1 Ki BMDMs stimulated with LZ.

In all panels, BMDMs were stimulated with LPS, LZ, LZNs, Z, or Ns as indicated. ELISA data are shown as ±SD from three independent experiments compared using two-way ANOVA: n.s. (p > 0.05) and ****p < 0.0001. Kinetic cell death experiments are representative of three or more independent experiments and presented as the mean ± SD of triplicate wells.