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. 2020 Mar 30;17:216–231. doi: 10.1016/j.omto.2020.03.017

Figure 3.

Figure 3

ZNF331 Was Lowly Expressed in Gliomas and Inhibited the Formation of Vascular Mimicry

(A) ZNF331 protein expression levels in NBT, LGGT, and HGGT. Data are expressed as mean ± SD (n = 12). Compared with the NBT group, ∗∗p < 0.01. Compared with the LGGT group, ## p < 0.01. (B) ZNF331 protein expression levels in NHAs and U87 and U251 cells. Data are expressed as mean ± SD (n = 3). Compared with the NHA group ∗∗p < 0.01. (C) Detecting the effects of knockdown and overexpression of ZNF331 on the proliferation ability of U87 and U251 cells. Data are expressed as mean ± SD (n = 3). Compared with the ZNF331 (–)-NC group, ∗∗p < 0.01. Compared with the ZNF331 (+)-NC group, ##p < 0.01. (D) Detecting the effect of ZNF331 on the migration and invasion ability of U87 and U251 cells. Data are expressed as mean ± SD (n = 3). Compared with the ZNF331 (–)-NC group, ∗p < 0.05 and ∗∗p < 0.01. Compared with the ZNF331 (+)-NC group, ##p < 0.01. The scale bar indicates 50 μm. (E) Detecting the effects of ZNF331 on the VM formation ability of U87 and U251 cells. Data are expressed as mean ± SD (n = 3). Compared with the ZNF331 (–)-NC group, ∗∗p < 0.01. Compared with the ZNF331 (+)-NC group, #p < 0.05 and ##p < 0.01. The scale bar indicates 50 μm. (F) Detecting the effects of knockdown and overexpression of ZNF331 regulates LAMC2 protein expressional levels in U87 and U251 cells, respectively. Data are expressed as mean ± SD (n = 3). Compared with the ZNF331 (–)-NC group, ∗∗p < 0.01 and ∗p < 0.05. Compared with the ZNF331 (+)-NC group, ##p < 0.01. (G) Expression levels of ZNF331 mRNA and protein are detected in U87 and U251 cells after knockdown of PABPC5. Data are expressed as mean ± SD (n = 3). Compared with the PABPC5 (–)-NC group, ∗p < 0.05 and ∗∗p < 0.01. (H) The expression levels of ZNF331 mRNA and protein are detected in U87 and U251 cells after knockdown and overexpression of HCG15. Data are expressed as mean ± SD (n = 3). Compared with the HCG15 (–)-NC group, ∗∗p < 0.01. Compared with the HCG15 (+)-NC group, #p < 0.05. (I) Expression levels of ZNF331 mRNA and protein in U87 and U251 cells after knockdown of PABPC5, knockdown of HCG15, and overexpression of HCG15. Data are expressed as mean ± SD (n = 3). Compared with the control group, ∗p < 0.05 and ∗∗p < 0.01. Compared with the PABPC5 (–) + HCG15 (–)-NC group, #p < 0.05 and ##p < 0.01. Compared with the PABPC5 (–) + HCG15 (+)-NC group, &p < 0.05 and &&p < 0.01.