Table 1.
The primer sequences of the RT-qPCR tested genes
| Gene | Primer forward (5′ to 3′) | Primer reverse (5′ to 3′) |
|---|---|---|
| Bmal1 (rat) | GGCTGTTCAGCACATGAAAAC | GCTGCCCTGAGAATTAGGTGTT |
| Clock (rat) | CTTCCTGGTAACGCGAGAAAG | GTCGAATCTCACTAGCATCTGAC |
| Per1 (rat) | GATGTGGGTGTCTTCTATGGC | AGGACCTCCTCTGATTCGGC |
| Per2 (rat) | CAGGTTGAGGGCATTACCTCC | AGGCGTCCTTCTTACAGTGAA |
| Cry1 (rat) | CACTGGTTCCGAAAGGGACTC | CTGAAGCAAAAATCGCCACCT |
| Cry2 (rat) | CACTGGTTCCGCAAAGGACTA | CCACGGGTCGAGGATGTAGA |
| Sirt1 (rat) | TGATTGGCACCGATCCTCG | CCACAGCGTCATATCATCCAG |
| Nampt (rat) | CCTGGTATCCAATTACAGTGGC | CCAAATGAGCAGATGCCCCTAT |
| Glut4 (rat) | ACACTGGTCCTAGCTGTATTCT | CCAGCCACGTTGCATTGTA |
| Pparg (rat) | GGAAGACCACTCGCATTCCTT | GTAATCAGCAACCATTGGGTCA |
| β-Actin (rat) | GGCCAACCGTGAAAAGATGACC | AACCCTCATAGATGGGCACAG |
| Bmal1 (mouse) | ACAGTCAGATTGAAAAGAGGCG | GCCATCCTTAGCACGGTGAG |
| Clock (mouse) | ATGGTGTTTACCGTAAGCTGTAG | CTCGCGTTACCAGGAAGCAT |
| Per1 (mouse) | GAATTGGAGCATATCACATCCGA | CCCGAAACACATCCCGTTTG |
| Per2 (mouse) | CTCCAGCGGAAACGAGAACTG | TTGGCAGACTGCTCACTACTG |
| Sirt1 (mouse) | ATGACGCTGTGGCAGATTGTT | CCGCAAGGCGAGCATAGAT |
| Nampt (mouse) | GCAGAAGCCGAGTTCAACATC | TTTTCACGGCATTCAAAGTAGGA |
| Glut4 (mouse) | GGACCGGATTCCATCCCAC | TCCCAACCATTGAGAAATGATGC |
| Pparg (mouse) | CTCCAAGAATACCAAAGTGCGA | GCCTGATGCTTTATCCCCACA |
| β-Actin (mouse) | GTGACGTTGACATCCGTAAAGA | GCCGGACTCATCGTACTCC |