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. 2020 Apr 25;17:132. doi: 10.1186/s12974-020-01801-9

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 6 — Effects of L-VGCC blockade on laser-induced CNV and expression of COX-2 and phospho-Iκbα in mice. Fundus in vivo images of vehicle-treated laser CNV fundus 5 days after induction of the model (a) and Zinc20267861 (10 μg) subconjunctival treated eyes (e). Arrows indicate laser burn spots. In vivo fluorescent angiography in vehicle-treated laser CNV animals 30 (b), 60 (c), and 120 (d) after subcutaneous injection of fluorescein sodium 1 mg/kg and same time points (f–h) for Zinc20267861 (10 μg) subconjunctival treated eyes. Arrows indicate fluorescein leakage areas. Ex vivo evaluation of the laser spot sizes in retinal pigment epithelia choroid-scleral complexes (RCSC) flat mounts in control (i) and Zinc20267861 (10 μg) treated eyes (j) counterstained with GSA-lectin. Immunofluorescent detection of COX-2 (k, l) and phospho-Iκbα (m, n) in RCSC flat mounts in control and Zinc20267861-treated eyes. Quantitative analysis of fluorescein leakage area over time in vivo (o). A one-way ANOVA test with Tukey multiple comparisons was used to determine statistical significance. ***p < 0.001; (n = 3). Ex vivo quantitative analysis of fluorescein-stained neovessels (n = 6) (p), retinal pigment epithelia disruption area (n = 6) (q), and GSA-lectin stained neovessels (n = 6) (r). Student t-test was used to determine statistical significance between two groups. *p < 0.05; **p < 0.01; ***p < 0.001