Fig. 3.

Grafted human lt-NES cell–derived cortical neurons establish monosynaptic connections with host neurons in the contralateral cortex of stroke-injured rats. (A) Brain slice showing GFP+ grafted neurons in ipsilateral and mCherry+ host neurons transduced by rabies virus in the contralateral somatosensory cortex. (B) Confocal images of mCherry+, GFP+ grafted cell expressing the corticocallosal projection neuron marker Satb2 (gray) in the transplant core. Nuclear staining (Hoechst, blue) is included in the merged panel. (Scale bar in B, 20 μm.)