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. 2020 Apr 15;117(16):9054–9063. doi: 10.1073/pnas.1920463117

Fig. 1.

Fig. 1.

αGalCer-mediated iNKT cell activation enhances self-reactive innate B cell responses in chronic inflammation. (A) Experimental scheme. Mice were immunized i.p. with 5 μg α-galactosylceramide (αGC) on day 1 and i.p. 2 μg IL-18 for days 1 to 10. To follow NP-specific B cell responses, 0.5 μg of NP-αGC was injected intravenously. (B) ELISA was used to measure total Ig concentrations from serum at the indicated time points (the statistical difference between IL-18 and IL-18 + αGalCer is shown). (C) OD of anti-DNA IgE, IgM, and IgG on day 12. (D) Anti-DNA IgG1, IgG2b, and IgG3 and the ratio of IgG2b vs. IgG1 on day 12. Two-way (B) or one-way ANOVA (C and D) was employed to assess statistical differences. *P < 0.05, **P < 0.001, ***P < 0.0001; ns, not significant. Data are representative of two to four experiments with three to five mice per group. Graphs display mean ± SEM.