Table 1.

Amino acid positions identified by proximity to the chromophore that contain S-A or F-Y substitutions (residues in bold)

Amino acid nos.			LWS						MWS			UVS
NoL1	JSR1	BVRh	A (4)	B (6)	C (2)	D (2)	E (3)	F (3)	G (2)	H (4)	I (4)	J (2)	K (1)
110	107	94	M3 L1	M6	M2	L2	M3	L2 V1	F2	Y3 F1	Y2 V1 L1	F2	F1
129	126	113	Y4	Y6	Y2	Y2	Y3	Y3	Y2	Y4	H2 F1 Y1	F1 Y1	F1
134	131	118	N4	N6	N2	N2	S3	S3	G2	A4	A2 S1 G1	A2	S1
194	191	178	Y4	Y6	Y2	Y2	Y3	Y3	Y2	Y4	F3 Y1	F2	Y1
205	202	189	T4	T6	T2	T2	T3	T3	F2	F3 Y1	F4	F2	F1
226	223	210	F4	F6	F2	Y2	Y3	F1 Y2	Y2	Y4	F2 Y2	W2	Y1
318	317	292	S4	S5 ?1	S2	S2	S3	S3	A2	A4	G3 A1	A2	A1

Amino acid sites are identified based on numbering in the NoL1 opsin of N. oerstedii (NoL1), the crystal structure of rhodopsin from the jumping spider Hasarius adansoni (JSR1; accession no. 6I9K_A), and bovine rhodopsin (BvRh; accession no. NP_001014890.1). Opsins are divided by spectral clades (LWS, MWS, and UVS) and subgroups (A to K). The number of sequences within each subgroup are indicated in the parentheses after each letter. Within each subgroup, all amino acids at that position are indicated, with the number of sequences encoding that amino acid indicated by subscript.