Table 1.

Statistics of cryo-EM data collection, processing, and model fitting

	EEEV	EEEV–Hp
Data collection and processing
Nominal magnification	38,000	81,000
Voltage, kV	300	300
Total electron dose, e−/Å2	32.05	32.07
No. of frames per movie	40	60
Exposure time, s	10	12
Frame exposure time, ms	250	200
Dose rate, e−⋅Å−2⋅s−1	3.20	2.67
Defocus range, μm	0.5–3.5	0.5–3.5
Pixel size, Å	1.58	1.73
Number of micrographs collected	696	1,581
Final particle images used for single particle reconstruction, no.	11,867	17,022
Symmetry imposed	Icosahedral	Icosahedral
Map resolution, Å (FSC = 0.143)
Unmasked	5.17	5.79
Masked	4.76	5.56
Sharpening B factor, Å2	−174	−305
Model fitting
Model used	—	E1–E2 heterodimer (PDB: 6MX4)
Fitting method	—	Rigid-body fitting using EMfit
Sumf* (%) value (EMfit)	—	27.58
Model-map correlation coefficient (Phenix)†	—	0.7440

*Sumf is the average density at the positions of the fitted atoms. The density was normalized by the highest density (100%) in the cryo-EM map (31, 32).

^†Model-map correlation coefficient was calculated using phenix.get_cc_mtz_pdb (58).