Fig. 3.

SP8 reduced the transcriptional activation activity of OsARF17. (A) Schematic diagrams of the effectors and reporters used in the dual-LUC experiments. The effectors BD, OsARF17-BD, and SP8 fused FLAG-tag. The reporters 35S: REN-5*gal pro:LUC plasmids. The BD domain can bind the 5*gal promoter. (B) The relative LUC activities were measured in N. benthamiana cells, using the combinations shown in A. The empty BD effector was used as a negative control. The LUC/REN ratio represents the relative LUC activity. (C) The effect of synthetic auxin (2,4-D or NAA) treatments on transgenic rice plants expressing the SP8 gene (SP8-13 and SP8-26) driven by the CaMV 35S promoter. The seedling shoots of SP8-13 and SP8-26 were immersed in 0.1 μm 2, 4-D, and 0.1 μm NAA culture solution in the dark and compared with 0.1% Triton X-100 treated rice controls for 10 d at 37 °C. Each treatment used at least 25 to 30 plants. Pictures were taken after 10 d of treatment. (Scale bar, 1 cm.) (D) Statistical analyses of primary root length. Values shown are the means ± SD of 3 biological replicates. Significant differences were identified using Fisher's least significant difference tests. At the top of columns, significant difference at *P ≤ 0.05 and **P ≤ 0.01, respectively. ns, no significant difference.