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. 2020 Apr 7;117(16):8850–8858. doi: 10.1073/pnas.1918759117

Fig. 2.

Fig. 2.

CtaI loads PHBA onto holo-CtaH. (A) MALDI-TOF spectral overlay of holo-CtaH loading reactions performed with PHBA and either CtaI or CtaD. The expected average masses of holo-CtaH and PHBA-S-CtaH are indicated. (B) HPLC absorbance profiles (250 nm) of reactions performed with NAC, PHBA, and CtaI. (C) Quantification of ATP hydrolysis products using a malachite green assay. Error bars represent the SD from the mean (n = 3). PPase, inorganic pyrophosphatase. (D) HPLC profiles of CtaI reactions with NAC. Red strikethrough indicates heat-inactivated enzyme; red asterisk, NAC-specific peak.