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. 2020 Apr 9;117(16):9064–9073. doi: 10.1073/pnas.1914505117

Fig. 1.

Fig. 1.

Qualitative immunofluorescence analysis of PHIP and focal adhesion proteins in U-251 stable cells. Images representing immunofluorescent detection of PHIP and pPXN (Y118) (A), TLN1 (B), ZYX (C), VCL (D), and F-actin (E) in U-251 transformants stably expressing anti-luc shRNA or anti-PHIP shRNA#1. DAPI staining was used to counterstain the nuclei (Scale bars: 20 µm.) White arrows point to the leading edge of cells. Quantification of the immunofluorescence results, including statistical analysis, is provided in SI Appendix, Fig. S3.