Fig. 2.

HF regulates cytokine responses in cells lacking GCN2. (A) FLS from wild-type and gcn2^−/− mice were pretreated with HF and/or proline and treated with TNF-α for 6 h. Transcript levels of target genes were quantified by qPCR. Results are representative of three independent experiments (means ± SD, n = 3); ***P < 0.001. (B) Summary of results from RNA-seq transcriptomics comparing HFol and TNF-α action in wild-type and GCN2-depleted K4 FLS. (Left) GCN2 depletion reduces HFol induction of canonical AAR genes (highlighted in green). (Center) GCN2 depletion has no significant effect on TNF-α–induced genes (highlighted in red). (Right) GCN2 depletion either has no effect, or enhances, HFol effects on TNFα induced genes. ****P < 0.0001, two-tailed Pearson correlation test.