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. 2020 Apr 21;15:2669–2683. doi: 10.2147/IJN.S249912

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© 2020 Li et al.

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Effects of ZnO NPs on mitochondrial density and mtDNA copy number in hiPSC-CMs. HiPSC-CMs were treated with ZnO NPs at 0, 25, 50, and 100 μM for 6 h. Representative fluorescence images of the cells stained with Mito-Tracker (green) for mitochondria mass and Hoechst 33342 (blue) for nucleus (A, Scale bar = 50 μm), and quantitative analysis of Mito-Tracker fluorescence intensity (B). mtDNA copy number was determined by qPCR (C). Data were presented as mean ± SE (n = 3). *Compared with control, P < 0.05.