Table 4.

Effect of different stocking densities and concentrations of dl-methionine supplementation on tissue lipid profile of broilers¹

Density	Normal		High			P-value
Methionine	100%	130%	100%	130%	SEM	Density	MET	Interaction
Grower
Breast
TC, mg/g protein	2.80	2.83	2.88	2.91	0.05	0.63	0.84	1.00
TG, mg/g protein	16.3	15.2	11.7	11.2	2.58	0.07	0.64	1.00
NEFA, μmol/g protein	4.91	5.81	4.85	4.63	0.52	0.09	0.37	0.13
Thigh
TC, mg/g protein	4.23	4.05	3.97	3.80	0.06	0.29	0.48	0.98
TG, mg/g protein	27.4	24.6	23.6	24.5	1.53	0.62	0.82	0.65
NEFA, μmol/g protein	10.1	10.5	8.69	9.02	0.79	0.28	0.78	0.98
Liver
TC, mg/g protein	12.8	13.2	12.3	14.3	0.74	0.72	0.19	0.40
TG, mg/g protein	25.9	30.1	24.5	28.6	2.28	0.50	0.05	1.00
NEFA, μmol/g protein	43.5	44.8	41.7	42.7	1.30	0.60	0.75	0.96
Finisher
Breast
TC, mg/g protein	4.83	4.39	4.99	4.81	0.25	0.21	0.17	0.55
TG, mg/g protein	25.0	18.1	17.9	24.9	3.85	0.92	0.90	0.09
NEFA, μmol/g protein	4.78ab	3.68b	4.75ab	5.67a	0.71	0.07	0.85	0.05
Thigh
TC, mg/g protein	3.75	3.82	3.81	3.69	0.06	0.82	0.88	0.54
TG, mg/g protein	19.9	26.6	24.9	29.4	3.52	0.34	0.18	0.80
NEFA, μmol/g protein	3.92	4.29	4.93	5.29	0.57	0.08	0.52	0.99
Liver
TC, mg/g protein	12.8	13.5	14.5	13.6	0.68	0.24	0.95	0.29
TG, mg/g protein	57.2	70.7	77.2	72.0	8.24	0.19	0.63	0.25
NEFA, μmol/g protein	32.6	38.3	37.8	43.7	4.28	0.09	0.07	0.98

¹Data are expressed as means (n = 10). The main effects (the stocking density and concentration of supplemental dl-methionine) were analyzed by two-way ANOVA and Duncan’s multiple range test was used to compare the treatment means. Means in the same row without a common letter differ significantly (P < 0.05).