Table 6.

Effect of different stocking densities and concentrations of dl-methionine supplementation on antioxidant status of broilers¹

Density	Normal		High			P-value
Methionine	100%	130%	100%	130%	SEM	Density	MET	Interaction
Grower
Plasma, nmol/mL
GSH	8.16b	5.97b	9.90a	10.5a	2.56	<0.01	0.25	0.19
GSSG	0.42a	0.32b	0.43ab	0.32b	1.34	0.71	<0.01	0.74
Liver, µmol/g
GSH	0.79ab	0.81a	0.60b	0.70ab	0.05	0.02	0.35	0.57
GSSG	0.074	0.059	0.064	0.063	0.013	0.75	0.34	0.36
Breast, µmol/g
GSH	0.38b	0.37b	0.47a	0.49a	0.04	<0.01	0.16	0.51
GSSG	0.030	0.027	0.030	0.033	0.008	0.40	0.99	0.43
Thigh, µmol/g
GSH	0.43c	0.54b	0.48bc	0.65a	0.06	<0.01	<0.01	0.29
GSSG	0.064ab	0.073a	0.052b	0.068ab	0.016	0.15	0.03	0.53
Finisher
Plasma, nmol/mL
GSH	7.92b	11.5a	10.3ab	10.3ab	1.87	0.57	0.05	0.03
GSSG	0.76ab	0.84a	0.69b	0.66b	0.92	<0.01	0.21	0.10
Liver, µmol/g
GSH	1.23	1.15	1.14	1.36	0.23	0.46	0.39	0.07
GSSG	0.20ab	0.26a	0.16b	0.18b	0.04	<0.01	0.09	0.47
Breast, µmol/g
GSH	0.672	0.716	0.648	0.701	0.08	0.64	0.24	0.91
GSSG	0.056a	0.051a	0.034b	0.023b	0.011	<0.01	0.15	0.67
Thigh, µmol/g
GSH	0.40ab	0.50a	0.28b	0.34b	0.08	<0.01	0.06	0.53
GSSG	0.066ab	0.077a	0.068ab	0.063b	0.001	0.16	0.48	0.09
MDA, µmol TEP equivalent/mg protein
Liver	167	157	161	155	12.2	0.81	0.56	0.89
Breast	28.1a	9.65b	12.5b	10.9b	2.97	0.03	<0.01	0.02
Thigh	26.5a	17.0b	21.6ab	14.3b	2.32	0.14	<0.01	0.66
Adipose tissue	872	687	555	596	132	0.17	0.60	0.43
Plasma FRAP, mmol Trolox equivalent/L
	10.1	10.7	10.4	9.1	0.77	0.66	0.45	0.23
Plasma amyloid A, ng/mL
	90.9	89.2	92.8	85.2	11.6	0.93	0.69	0.80
Plasma corticosterone, ng/mL
	364	405	345	396	47.7	0.81	0.38	0.93
Liver protein carbonyl, nmol/mg protein
	3.88	3.85	3.73	4.42	0.36	0.57	0.34	0.33

¹Data are expressed as means (n = 10). The main effects (the stocking density and concentration of supplemental dl-methionine) were analyzed by two-way ANOVA and Duncan’s multiple range test was used to compare the treatment means. Means in the same row without a common letter differ significantly (P < 0.05).