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. 2020 Mar 23;98(4):skaa092. doi: 10.1093/jas/skaa092

Table 7.

Effect of different stocking densities and concentrations of dl-methionine supplementation on antioxidant enzymes activity of broilers1

Density Normal High P-value
Methionine 100% 130% 100% 130% SEM Density MET Interaction
Grower
 Breast, U/mg protein
  GR 3.44 3.53 4.72 4.08 0.25 0.08 0.59 0.47
  GPx 18.1 18.2 16.3 16.6 0.58 0.14 0.85 0.92
  GST 42.6 49.1 44.6 43.8 1.42 0.57 0.32 0.21
  SOD 0.94 0.93 1.00 0.50 0.10 0.36 0.20 0.21
 Thigh, U/mg protein
  GR 4.23 3.87 4.44 4.57 0.21 0.31 0.80 0.59
  GPx 34.1 28.7 27.7 34.6 1.19 0.92 0.73 0.01
  GST 69.1a 62.3ab 58.0b 56.5b 1.70 0.01 0.20 0.41
  SOD 0.43ab 0.19b 0.26b 0.62a 0.05 0.12 0.47 <0.01
 Liver, U/mg protein
  GR 12.8 11.9 10.7 10.7 0.38 0.04 0.55 0.55
  GPx 106 127 110 102 6.53 0.43 0.62 0.30
  GST 205ab 223a 211ab 188b 5.04 0.13 0.78 0.04
  SOD 0.39ab 0.36ab 0.46a 0.23b 0.03 0.64 0.04 0.12
 Adipose tissue, U/mg protein
  GR 20.6 17.3 20.7 20.2 0.65 0.25 0.18 0.28
  GPx 176b 189ab 184ab 209a 4.94 0.14 0.05 0.54
  GST 101b 158a 162a 148 ab 9.25 0.93 0.22 0.06
  SOD 2.22 1.91 2.46 1.37 0.27 0.81 0.25 0.52
Finisher
 Breast, U/mg protein
  GR 4.11 4.23 5.49 4.34 0.28 0.20 0.37 0.27
  GPx 20.2 21.6 22.1 19.7 1.02 0.98 0.82 0.37
  GST 34.1 40.3 40.4 34.0 1.59 1.00 0.98 0.05
  SOD 0.21 0.30 0.23 0.19 0.02 0.33 0.49 0.13
 Thigh, U/mg protein
  GR 2.82b 4.18a 4.10a 4.36a 0.21 0.07 0.04 0.16
  GPx 13.7 15.4 14.5 16.2 0.72 0.58 0.24 0.99
  GST 68.6a 68.5a 69.1a 51.1b 2.07 0.02 0.01 0.02
  SOD 0.22 0.17 0.14 0.21 0.02 0.65 0.88 0.26
 Liver, U/mg protein
  GR 15.3 15.2 15.6 13.1 0.43 0.29 0.13 0.17
  GPx 24.5 25.8 26.6 22.9 0.71 0.78 0.39 0.09
  GST 208ab 204 ab 218a 190b 4.29 0.81 0.06 0.15
  SOD 0.72 0.67 0.91 0.86 0.06 0.09 0.65 0.99
Adipose tissue, U/mg protein
  GR 8.83 10.3 13.0 9.19 0.77 0.53 0.29 0.19
  GPx 118 124 127 139 3.95 0.15 0.23 0.75
  GST 23.5b 64.4a 40.5ab 29.0b 5.18 0.32 0.12 <0.01
  SOD 2.21 2.66 1.34 1.85 0.34 0.26 0.51 0.97

1Data are expressed as means (n = 10). The main effects (the stocking density and concentration of supplemental dl-methionine) were analyzed by two-way ANOVA and Duncan’s multiple range test was used to compare the treatment means. Means in the same row without a common letter differ significantly (P < 0.05).