Table 1.
Primer sequences for the in-house designed multiplex PCR
| Target organism and strain | Primer sequence | Location of primer gene sequence | Size of PCR product | |
|---|---|---|---|---|
|
M. avium subsp. paratuberculosis MAP4 CP005928.1 |
Forward | 5´- CGCGCGTACC TGACAAAAC - 3´ |
562055 - 562037 |
187 bp |
| Reverse | 5´- TCACCCTGAC ACTGACAGACA - 3´ |
561869 - 561889 |
||
|
M. bovis strain SP38 CP015773.1 |
Forward | 5´- GATGGTGGAA CACGACCACT - 3´ |
4138314 - 4138333 |
571 bp |
| Reverse | 5´- TTGATCGACC GTTCCGGTTT - 3´ |
4138865 - 4138884 |
||
|
M. smegmatis MC2 155 CP009494.1 |
Forward | 5´- ACCATGTCTAT CTCAGTGTGCT - 3´ |
3877883 - 3877904 |
628 bp |
| Reverse | 5´- ACGCTCGAGGT CCACTACAA - 3´ |
3878510 - 3878491 |
||