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. Author manuscript; available in PMC: 2020 Oct 23.
Published in final edited form as: J Cell Sci. 2020 Feb 27;133(8):jcs238352. doi: 10.1242/jcs.238352

Figure 2. CEP85 interacts functionally with PLK4.

Figure 2

(A). Domain overview of human CEP85. cc coiled-coil. Vectors that were used in this work are shown by green lines. (B-D). Detection of expressed FLAG-CEP85 WT, Q640A+E644A mutant or fragments coimmunoprecipitating with Myc- PLK4. (E-F). U-2 OS cells expressing FLAG or the siRNA-resistant FLAG-CEP85 transgene were transfected with control or CEP85 siRNA for 72 h. (E). The graph indicates the percentage of cells with four centrioles at normal serum conditions (n = 100, three independent experiments). (F). The graph indicates the percentage of the percentage of wound area recovered at low serum conditions (n = 3/experiment, three independent experiments). Two-tailed t-test was performed for all p-values (**p < 0.01, *p <0.05).