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. Author manuscript; available in PMC: 2020 Jul 2.
Published in final edited form as: Lab Invest. 2020 Jan 2;100(5):777–785. doi: 10.1038/s41374-019-0365-z

Figure 2. Overexpression of OGT attenuates TRA-8 induced apoptosis in sensitive pancreatic cancer cells.

Figure 2.

A) BxPC-3, and B) MiaPaCa-2 cells were transfected with OGT expressing vector or control vector. Stable clones were selected by neomycin. a. Protein O-GlcNAcylation and OGT expression, as determined by Western blot analysis using specific antibodies. The expression of ß-actin was used as a loading control. Representative blots from three independent experiments are shown. b. TRA-8 induced apoptosis. Cells were exposed to TRA-8 (1 μg/ml) for 24 hours, and apoptosis were determined by flow cytometry (n=3, ***p<0.001).