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. Author manuscript; available in PMC: 2020 Jul 2.
Published in final edited form as: Lab Invest. 2020 Jan 2;100(5):777–785. doi: 10.1038/s41374-019-0365-z

Figure 3. Downregulation of OGT promotes TRA-8-induced apoptosis in resistant pancreatic cancer cells.

Figure 3.

A) PANC-1, and B) S2VP10 cells were infected with lentiviruses carrying scrambled shRNA (shScr) or shRNA for OGT (shOGT), and selected by puromycin. a. Protein O-GlcNAcylation and OGT expression, as determined by Western blot analysis using specific antibodies. The expression of ß-actin was used as a loading control. Representative blots from three experiments are shown. b. TRA-8 induced apoptosis. Cells were exposed to TRA-8 (1 μg/ml) for 24 hours, and apoptosis were determined by flow cytometry (n=3, ***p<0.001).