Table-3.
Correlation between the HRM melting temperature, the cluster numbers according to the ScreenClust software, and the sequencing results.
| Number | Sample ID | HRM melting temp. | Screen plot cluster | Sequencing result |
|---|---|---|---|---|
| 1 | 1V | 78.24 | CLUSTER 2 | Mass |
| 2 | 2V | 78.36 | CLUSTER 1 | 4\91 |
| 3 | 3V | 77.79 | CLUSTER 1 | CR 88 variant strain very close to 4\91 |
| 4 | 4V | 78.09 | CLUSTER 1 | 1\96 very close to 4\91 |
| 5 | 5V | 78.39 | CLUSTER 2 | H-120 very close to Mass |
| 6 | T1 | 78.81 | CLUSTER 2 | Mass |
| 7 | T2 | 78.39 | CLUSTER 2 (borderline of cluster 2) | Variant II – mismatch |
| 8 | T3 | 78.36 | CLUSTER 2 | Mass |
| 9 | T4 | 78.36 | CLUSTER 2 | Mass |
| 10 | T5 | 78.36 | CLUSTER 2 | Mass |
| 11 | L1 | 78.90 | CLUSTER 3 | Variant II |
| 12 | L2 | 78.45 | CLUSTER 2 | Mass |
| 13 | K1 | 79.05 | CLUSTER 3 | Variant II |
| 14 | K2 | 78.39 | CLUSTER 2 | Mass |
| 15 | 20417 | 78.90 | CLUSTER 3 | Variant II |
| 16 | 21417 | 78.75 | CLUSTER 3 | Variant II |
| 17 | 22417 | 78.60 | CLUSTER 3 | Variant II |
| 18 | 51216D | 78.81 | CLUSTER 3 | Variant II |
| 19 | 61216D | 78.75 | CLUSTER 2 (borderline of cluster 3) | Variant II – mismatch |
| 20 | 101216D | 78.30 | CLUSTER 1 | 4\91 |
| 21 | 6417P | 78.30 | CLUSTER 3 | Variant II |
| 22 | 7417P | 78.51 | CLUSTER 2 | Mass |
| 23 | 8417P | 78.75 | CLUSTER 3 | Variant II |
| 24 | 072017P | 78.69 | CLUSTER 4 | Q1 |
| 25 | 0605P | 78.45 | CLUSTER 2 | Variant II – mismatch |
| 26 | D10117 | 77.45 | CLUSTER 1 | 4\91 |
| 27 | 7P6 | 77.49 | CLUSTER 1 | 4\91 |
HRM: High-resolution melting