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. 2020 Feb 8;69(5):717–730. doi: 10.1007/s00262-020-02497-9

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — The proportion of NKT cells increases in responders after 1 month of anti-PD1 treatment. Gating strategies of a CD3⁺CD56⁺ and CD3brightCD56⁺ NKT cells from lymphocytes, b CD3⁺CD4⁺ and CD3⁺CD8⁺ T cells from total T cells, c CD56dim and CD56bright NK cells from the total lymphocyte population before treatment (pre), at 1 month (1mo) and at 3 months (3mo) of anti-PD1 therapy. Green circles represent the complete responders (CR), black circles represent responders (R) and black triangles represent non-responders (NR). The statistical difference between time points within same cohort was calculated with Students t test