Figure 7.

GO enrichment analysis and KEGG pathway analysis were used to explore the possible mechanism of activated ADMSC for treatment of intestinal IR injury. (a) GO enrichment analysis revealed that differentially expressed mRNAs were involved in “biological process,” “cellular component,” and “molecular function” processes. (b) Deregulated mRNAs participated in multiple biological functions like “neutrophil chemotaxis,”, “inflammatory response”, “chemotaxis” and “defense response”. (c) KEGG pathway analysis showed that several pathways (NF-κB-P65, MAPK-ERK, and PI3K-AKT pathways) were involved in activated ADMSC for treatment of intestinal IR injury. (d) Results from western blot showed that activated ADMSC inhibited phosphorylation of P65, ERK, and AKT that were induced by IR injury. (e, f) Silencing of COX-2 impaired the effect of activated ADMSC on inhibition of the seven mRNA expressions and inaction of NF-κB-P65, MAPK-ERK, and PI3K-AKT pathways in intestinal IR-injured tissue.