Fig. 8.

miR-135b activates AKT/mTOR pathway to elevate the tumorigenicity of PCSCs. a Macroscopic observation of tumor size. b Measurement of tumor volume in nude mice with vernier caliper. c Determination of tumor mass in nude mice by weighing method. d Immunohistochemistry was performed to detect positive expression of JADE-1, phosphorylated AKT and phosphorylated mTOR (×400); n = 6, *p < 0.05 vs. the agomir-miR-135b + perifosine group; ^#p < 0.05 vs. the antagomir-NC group; ^&p < 0.05 vs. the agomir-miR-135b + DMSO group. n = 6. The measurement data were expressed as mean ± standard deviation. Unpaired student’s t-test was used to compare unpaired data between the two groups. Repeated measurement ANOVA was used to compare the data among multiple groups at different time points, followed by the Bonferroni post hoc test