Table 1.

RT-qPCR primer sequences

miRNA/gene	Primer sequence
miR-135b	F: 5′-CTGTGGCCTATGGCTTTTCAT-3′
	R: 5′-GCTCGCCCCTCACTGTAG-3′
U6	F: 5′-CGCTTCGGCAGCACATATAC-3′
	R: 5′-AAATATGGAACGCTTCACGA-3′
JADE-1	F: 5′- AGGGATTAAAGTGCTCCCCC-3′
	R: 5′-TGGCGTTTGGCTTGTTATGG-3′
GAPDH	F: 5′-AATGGGCAGCCGTTAGGAAA-3′
	R: 5′-GCGCCCAATACGACCAAATC-3′
CD24	F: 5′-CCCCACCTTGCCTGCG-3′
	R: 5′-AAATCTGCGTGGGTAGGAGC-3′
CD44	F: 5′-TTACAGCCTCAGCAGAGCAC-3′
	R: 5′-TGACCTAAGACGGAGGGAGG-3′
ESA	F: 5′-TGGTTTCAGGGGGCTGTTGT-3′
	R: 5′-TCGGCCGTCTCTACGTCCTC-3′
Sox-2	F: 5′-GCCTGGGCGCCGAGTGGA-3′
	R: 5′-GGGCGAGCCGTTCATGTAGGTCTG-3′
Oct-4	F: 5′-GCTCGAGAAGGATGTGGTC-3′
	R: 5′-ATCCTCTCGTTGTGCATAGTCG-3′
Nanog	F: 5′-GCTGAGATGCCTCACACGGAG-3′
	R: 5′-TCTGTTTCTTGACTGGGACCTTGTC-3′
ALDH1	F: 5′-GCACGCCAGACTTACCTGTC-3′
	R: 5′-CCTCCTCAGTTGCAGGATTAAAG-3′
Slug	F: 5′-CGAACTGGACACACATACAGTG-3′
	R: 5′-CTGAGGATCTCTGGTTGTGGT-3′

RT-qPCR reverse transcription quantitative polymerase chain reaction, microRNA-135b miR-135b, JADE-1 jade family PHD finger 1, GAPDH glyceraldehyde 3-phosphate dehydrogenase, ESA electrical stimulation for analgesia, F forward primer, R reverse primer, Sox-2 SRY related HMG box-2, Oct-4 Octamer-4, ALDH1 aldehyde dehydrogenase isoform 1