FIGURE 10:

Aβ42 residues are potentially targeted by PI3K. (A) Serine and tyrosine residues in Aβ42 sequence subject to phosphorylation. (B) Images of p-Ser (green) and DAPI (magenta) stainings in 15-d-old adult brains of the corresponding genotypes (see Figure 1 legend). Equivalent images of brains stained for p-Tyr (green) are shown in the right panels. Note the increment of p-Ser, but not p-Tyr, immunosignal in PI3K/Aβ42 brains. (C) Images of p-Ser26 (green), p-Ser8 (red), and DAPI (magenta) immunostaining in 15-d-old adult brains of the corresponding genotypes. (D) Quantification of pixel intensities in 10 images from three brains as those shown in panels B and C. One-way ANOVA significances are indicated with ***, p < 0.001. Scale bar is 10 µm (B) and 50 µm (C).