FIGURE 8:

Eisosome mutations do not impair endocytosis or cell integrity. (A) Wild-type and mutant cells were grown in SD_comp + 1 M sorbitol. The cells of the midlog yeast cultures were moved into SD_comp pH 4 medium and after 10 min the cells were stained with PI and analyzed by flow cytometry. (B) Microscopy pictures of Fur4-GFP expressing cells (WT, SEY6210 pJK19; nce102∆, AMY41 pJK19) in the presence or absence of 50 mM Tris, pH 8, were quantified by determining the signal at the plasma membrane of Fur4 relative to the total signal. The graph shows the result of ∼50 cells for each strain and condition. The black line indicates the median of the data set.