Representative western blots and densitometry analysis of the BBB tight junction proteins ZO-1, occludin, claudin-5 and JAM-1 in brain microvessels isolated from young (A), aged (B) wild type mice, and (C) TgSwDI mice. Statistical analysis was determined by Student’s t-test (n=5–7 mice). All values were normalized to CTR (1.0). Data are presented as box-and-whisker plots representing median and IQR, with minimum and maximum values. *P < 0.05, and **P < 0.01.