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. 2020 Apr 22;13:3389–3399. doi: 10.2147/OTT.S249662

Figure 4.

Figure 4

miR-934 directly targeted and inhibited PROX1 expression. (A) The correlation analysis between miR-934 and PROX1 performed using expression data from the TCGA database. (B) Western blot analysis  used to detect the expression of PROX1 in different miR-934 expression cell lines. GAPDH was used as an internal control. (C) The relative gray level of Western blot was quantified and analyzed. (D) PROX1 mRNA expression level in cells transfected with miR-934 mimics or inhibitor was measured by qRT-PCR. (E) Potential binding targets of miR-934 to PROX1 predicted by the targetScan database. (F) Dual-luciferase reporter assay  used to verify the direct binding of miR-934 to PROX1. *P < 0.05, **P < 0.01, ***P < 0.001, ns: not significant.