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. 2020 Apr 22;13:3389–3399. doi: 10.2147/OTT.S249662

Figure 5.

Figure 5

PROX1 is necessary for miR-934 to exert its oncogenic function. (A) Western blot used to detect the transfection efficiency in Patu-8988 cells with miR-934-nc, miR-934-mimics and miR-934-mimics+PROX1 plasmid. (B) The gray level was quantified by imageJ. (C) Transfection efficiency measured by qRT-PCR. (D) CCK8 assay used to detect cell viability of pancreatic cancer cell in miR-934-nc, miR-934-mimics and miR-934-mimics+PROX1 group. (E) Left panel: colony formation assay to detect cell proliferation capacity. Right panel: the colony number was quantified using imageJ. (F, G) Left panel: wounding healing and transwell assays to determine the migration ability of pancreatic cancer in miR-934-nc, miR-934-mimics and miR-934-mimics+PROX1 group. Right panel: the migration rate and migrated cell number were quantified and analyzed by imageJ. *P < 0.05, **P < 0.01.