FIGURE 6:

Checkpoint kinase Rad53 modulates Hof1 levels. (A) Anti-HA Western blot of WT and hof1/hof1 strains with Rad53-HA that were incubated with 0.02% MMS for 90 min (MMS), resuspended in YPD, and further incubated for indicated times (2 and 6 h). Untreated cells (0) were included as controls. (B) Western blot of Hof1-HA in WT and a RAD53 deletion strain with and without a 90-min 0.02% MMS treatment. Hof1-HA was probed using an anti-HA antibody, and b-tubulin was a loading control. Band intensities relative to WT untreated are shown. Two-tailed t test; n = 3; **P < 0.01.