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. 2020 Apr 21;26(15):1758–1774. doi: 10.3748/wjg.v26.i15.1758

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©The Author(s) 2020. Published by Baishideng Publishing Group Inc. All rights reserved.

This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial.

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Mdivi-1 treatment protects cell viability and decreases apoptosis in Caco-2 hypoxia/reoxygenation cells. A: Cell viability was detected by the CCK8 method (n = 6); B: Representative Western blotting of cleaved caspase-3 protein expression in Caco-2 cells (n = 3); C: Effect of mdivi-1 on hypoxia/reoxygenation-induced cell apoptosis detected by TUNEL assay (scale bar = 100 μm); D: TUNEL positive cell ratio in hypoxia/reoxygenation-induced cell apoptosis (n = 3). ^aP < 0.05, ^bP < 0.01, ^cP < 0.001 compared with the control group; ^dP < 0.05, ^eP < 0.01 compared with the hypoxia/reoxygenation group. c-caspase 3: Cleaved caspase 3; H/R: Hypoxia/reoxygenation; TUNEL: Terminal-deoxynucleotidyl Transferase Mediated Nick End Labeling; DAPI: 4',6-diamidino-2-phenylindole.