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(A) Mouse Spast locus with targeted region in exon 5. Bases in red denote the PAM (protospacer adjacent motif) sequences and in green the regions in exon 5 targeted by the gRNAs. (B, C, D) Deletion in NSC34 cells was confirmed by (B) Western blot analysis, (C) immunofluorescence staining, and (D) real-time quantitative RT-PCR. Paired t test, ***P < 0.001. (E) Deletions identified in the KO cells. Scale bar: 10 μm.
