Table 1.

Clinical and Genomic Characteristics of NSCLC Cases with ALK Internal Rearrangements

	ALK Rearrangements in NSCLC Detected by CGP of DNA
	ALK Internal Inversion Alone	ALK Internal Inversion + Co-Occurring ALK Rearrangement/Fusion	ALK Internal Deletion Alone	ALK Internal Deletion + Co-Occurring ALK Rearrangement/Fusion
# cases	6 (6 tissue, 0 ctDNA)	19 (18 tissue, 1 ctDNA)	3 (3 tissue, 0 ctDNA)	5 (3 tissue, 2 ctDNA)
Median age, years (range)	60.5 (23–82)	63 (49–83)	55 (33–66)	55 (38–58)
Gender M:F	1:5	7:12	2:1	2:3
ALK breakpoints	introns 8/19 (n=2) introns 11/18 introns 15/19 (n=2) introns 17/19	introns 1/19 (n=7) introns 3/19 (n=2) introns 4/19 (n=2) introns 8/19 introns 11/18 (n=2) introns 11/19 introns 12/17 introns 14/19 introns 15/19 introns 17/18	introns 1/18 introns 3/19 introns 12/19	introns 1/19 introns 3/19 introns 5/19 (n=3)
Co-occurring ALK rearrangement/fusion	N/A	EML4-ALK fusion (n=15) CLTC-ALK fusion CLIP4-ALK fusion ALK intron 19/MPV17 rearrangement ALK intron 19/intragenic space rearrangement	N/A	EML4-ALK fusion ALK-EML4 reciprocal only TYW1B-ALK fusion ALK intron 19/HDAC8 ALK intron 19/intragenic space rearrangement
# with other drivers present	1 (EGFR L858R)	1 (MET amplification)	0	0
ALK FISH/IHC status	ALK+ FISHa ALK+ IHCb ALK FISH negative (n=2)c, d Not performed or unknown (n=2)	NE	ALK FISH negativea ALK+ FISHeNot performed or unknown	NE
ALK rearrangement status on RNA	EML4-ALK (n=2) Not performed or unknown (n=4)	NE	NE*	NE

Notes: a: FISH testing performed by NeoGenomics; b: IHC testing performed by Genepath, clone ALKO1; c: FISH testing performed by Genepath; d: FISH testing performed by Integrated Oncology; e: FISH testing performed by Response Genetics. *For 3/3 non-NSCLC cases with ALK internal deletions in DNA RNA testing showed only an ALK deletion and did not detect the presence of an ALK fusion.

Abbreviations: N/A: not applicable, NE: not evaluated for this study.