Table 1.
Bacterial Strains and Plasmids Used in This Study
| Bacterial Strains or Plasmids | Relevant Characteristics | Source or Reference |
|---|---|---|
| Bacterial Strains | ||
| E. coli DH5α | F-, φ80dlacZΔM15, Δ(lacZYA-argF)U169, deoR, recA1, endA1, hsdR17(rk-, mk+), phoA, supE44, λ-, thi-1, gyrA96, relA1 | Laboratory stock |
| E. coli C600 | F- tonA21 thi-1 thr-1 leuB6 lacY1 glnV44 rfbC1 fhuA1 λ- | Laboratory stock |
| E. coli ATCC 25922 | Quality control strains for antimicrobial susceptibility | Laboratory stock |
| E. coli SHP45 | Isolated from pig with colistin resistance plasmid | (Liu et al, 2015)1 |
| Plasmids | ||
| pHNSHP45 | mcr-1-harboring IncI2-type plasmid | (Liu et al, 2015)1 |
| pCas9 (Addgene,42876) | Cmr, pSC101 ori, cas9 expression plasmid | (Su et al, 2016)13 |
| pUC19 | Ampr, pUC ori | Laboratory stock |
| pCas9-m1 | Cmr, pCas9 cloned with sgRNA1 targeting mcr-1 | In this study |
| pCas9-m2 | Cmr, pCas9 cloned with sgRNA2 targeting mcr-1 | In this study |
| pUC19-mcr-1 | Ampr, pUC19-mcr-1 derivative with mcr-1 gene | In this study |
Notes: E. coli, Escherichia coli; Ampr, ampicillin-resistant; Cmr, chloramphenicol-resistant.