Table 2.

Primers Used in This Study

Primer Name	Primer Sequences (5ʹ-3ʹ)
sgRNA1-F	AAACAAAGCTGTTTGATGTCACCGG
sgRNA1-R	AAAACCGGTGACATCAAACAGCTTT
sgRNA2-F	AAACCGACATTATTAAAGCTGTTTGATGTCACCGG
sgRNA2-R	AAAACCGGTGACATCAAACAGCTTTAATAATGTCG
DR-JD-F	CACGCATTGATTTGAGTCAG
DR-JD-R	GGTGATGTCGGCGATATAGG
mcr-1-JD-F	TCGCGGCATTCGTTATA
mcr-1-JD-R	GGTGGCGTTCAGCAGTC
SacI-mcr-1-F	ataaaaGAGCTCCGAAGCACCAAGACATCAA
SacI-mcr-1-R	aaaataGAGCTCATACGGCATAACAAACCCC
mcr-1-qPCR-F	ACACTTATGGCACGGTCTATG
mcr-1-qpcr-R	GCACACCCAAACCAATGATAC
16S-qPCR-F	AAGTTAATACCTTTGCTCATTGAC
16S-qPCR-R	GCTTTACGCCCAGTAATTCC
M13-F	AGCGGATAACAATTTCACACAGGA
M13-R	CGCCAGGGTTTTCCCAGTCACGAC

Note: The italics are the protective bases, and the bold indicates the enzyme cutting site.