Fig. 2.

Downregulation of CDKN2B-AS1 inhibits lipid uptake and accumulation in macrophage-derived foam cells, and promotes mRCT. a & b, The expression of ABCA1, ABCG1 and CD36 proteins (normalized to β-actin) in THP-1 macrophage-derived and HPM-derived foam cells determined by Western blot analysis. c &d, Dil-oxLDL uptake of foam cells detected under a fluorescence microscope (× 400). e & f, The cholesterol efflux rates measured using a liquid scintillation counter with ApoAI as the acceptor. g & h, The intracellular lipid accumulation detected by oil O red staining (× 200). * p < 0.05 vs. the sh-NC group. ^#p < 0.05 vs. the oe-NC group. All the experiments were repeated 3 times independently, and the data comparisons between multiple groups were performed using one-way analysis of variance. oe-NC, cells transduced with LV5-GFP empty vector; oe-CDKN2B-AS1, cells transduced with LV5-GFP-CDKN2B-AS1; sh-NC, cells transduced with pSIH1-H1-copGFP-sh-NC; sh-CDKN2B-AS1, cells transduced with pSIH1-H1-copGFP-sh-CDKN2B-AS1.