Table 8.
Gene | Primer sequences |
---|---|
CDKN2B-AS1 | F: 5′-TCATCATCTCATCATCATC-3′ |
R: 5′-TGCTTCTGTCTCTTCATA-3′ | |
CDKN2B | F: 5′-TCAGTCGGCTTCCGAGGTA-3′ |
R: 5′-GTTGCGTGGTTCAATGCC-3′ | |
CTCF | F: 5′-GAACCCATTCAGGGGAAAAGC-3′ |
R: 5′-TCGCAAGTGGACACCCAAATC-3′ | |
GAPDH | F: 5′-ATGGAGAAGGCTGGGGCTC-3′ |
R: 5′-AAGTTGTCATGGATGACCTTG-3′ |
Note: CDKN2B-AS1, cyclin-dependent kinase inhibitor 2B antisense noncoding RNA; CDKN2B, cyclin-dependent kinase inhibitor 2B; CTCF, CCCTC-binding factor; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; RT-qPCR, reverse transcription quantitative polymerase chain reaction; F, forward; R, reverse.