Fig. 2.

LCL transcriptome after AHR activation (3-MC treatment) or inhibition (GNF-351 treatment). (A) A total of 1865 genes was differentially expressed after 3-MC treatment at a FDR <0.05, but only 16 of those genes were differentially expressed by at least 2-fold. More genes had expression that increased after 3-MC treatment than those that decreased. (B) A total of 3664 genes was differentially expressed after GNF-351 treatment at a FDR <0.05, but only 64 of those genes were differently expressed by at least 2-fold. (C) The figure shows a heatmap for the 69 genes that were differentially expressed by at least 2-fold with a FDR <0.05 after either 3-MC or GNF-351 treatment, but their degree of differential expression was variable across LCLs. (D) The SNP-dependent effect of rs2470893 after 3-MC treatment was determined by both qRT-PCR and RNA-seq in the samples that were used for RNA-seq and represented in violin plot. Each group contained four lymphoblastoid cell lines. P values were calculated using a two-tailed Student’s t test: *P < 0.05; ** P < 0.01; ***P < 0.001.