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. Author manuscript; available in PMC: 2020 Apr 27.
Published in final edited form as: ACS Chem Neurosci. 2019 Jun 19;10(8):3671–3681. doi: 10.1021/acschemneuro.9b00248

Figure 4.

Figure 4.

Desmosterol replaces cholesterol in Dhcr24−/−neurons in a time-dependent manner. WT (Dhcr+/+), Dhcr24-heterozygous (Dhcr24±), and Dhcr24-deficient (Dhcr24−/−) neurons were cultured in defined cholesterol-free medium and cholesterol (A) and desmosterol (B) levels were assayed at DIC3 and DIC6. Values correspond to mean ± SEM of 8–12 replicates. In all panels, statistical significance between individual groups is denoted by asterisks (****p < 0.0001). Note that in Dhcr24−/− neurons, cholesterol is fully replaced by desmosterol.