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. Author manuscript; available in PMC: 2020 Apr 27.
Published in final edited form as: ACS Chem Neurosci. 2019 Jun 19;10(8):3671–3681. doi: 10.1021/acschemneuro.9b00248

Figure 6.

Figure 6.

Astrocytes release more cholesterol than neurons. (A) Total cholesterol detected in the medium over 3 days in culture. Both neuronal and astroglial cells were cultured in a defined cholesterol-free medium. (B) Analyzed media were collected at DIC6 and reflect the release of cholesterol between DIC3 and DIC6. Cholesterol levels were normalized to the total cell count at DIC6. Values correspond to the mean ± SEM of 3 replicates. Note that astrocytes release 60% more cholesterol than neurons. (C) Increase in total cholesterol in neurons and astrocytes over a 3 day period. Values correspond to the mean ± SEM of 8 replicates. Note that neurons accumulated 17-fold more cholesterol than astrocytes over the 3 day period. (D) Ratio of accumulated over released cholesterol over a 3 day period. Note that neurons retain the vast majority of their newly synthesized cholesterol, while astroglial cells release more cholesterol than they accumulate. Values correspond to the mean ± SEM of 8 replicates. In all panels, statistical significance is denoted by asterisks (*p < 0.05, ***p < 0.001; ****p < 0.0001).