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. 2020 Apr 27;11(4):292. doi: 10.1038/s41419-020-2491-3

Fig. 1. Generation and confirmation of INPP4B knockout A549 cell lines by Crispr-Cas9 gene editing.

Fig. 1

a The gRNA sequence targeting the 13th exon of wild type (WT) INPP4B allele is highlighted with red frames and aligned with two INPP4B mutant alleles carrying reading frame shift mutations created by CRISPR-Cas9 editing in lung adenocarcinoma A549 cells, blank regions indicate the deleted nucleotides. b Sanger-sequencing chromatograms of the gRNA targeting regions from the two INPP4B mutant alleles created by Crispr-Cas9 technology, the nucleotide sequence is shown under each chromatogram. c Complete loss of INPP4B protein expression in Crispr-INPP4B A549 cells was confirmed. Note: the INPP4B knockout cells are a mixture of clone 7 and clone 9. d The expression of a 3xFlag tagged Crispr-resistant INPP4B was verified in Crispr-INPP4B A549 cells.