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. 2020 Apr 27;11(4):292. doi: 10.1038/s41419-020-2491-3

Fig. 5. IR irradiation triggers INPP4B nuclear translocation in A549 cells.

Fig. 5

a A549 cells stably transfected with pEGFP-C1-INPP4B plasmid were irradiated with 10 or 20 Gy gamma-ray, the sub-cellular localization of GFP-INPP4B protein was observed by fluorescence microscopy at the indicated recovery time, the representative pictures were taken at magnification ×400. b Quantitative analysis of nuclear redistribution of GFP-INPP4B. Image J software was employed to quantify the nuclear signal intensity of GFP-INPP4B, as well as the whole cellular signal of GFP-INPP4B with normalization to the signal of GFP-INPP4B measured in the untreated cells. The proportion of nuclear GFP-INPP4B signal was calculated by the normalized nuclear GFP-INPP4B signal versus the normalized whole cellular GFP-INPP4B signal. At least 100 cells were measured under each condition. The average proportion of nuclear GFP-INPP4B signal in each condition was plotted in b. An error bar stands for standard deviation of nuclear INPP4B signal.