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. 2020 Apr 27;10:7075. doi: 10.1038/s41598-020-64042-z

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Heterogenetic parabiotic pairing decreased inflammation in dystrophic skeletal muscle. (A) Necrotic areas in the gastrocnemius muscles were identified by mouse IgG staining (red). The nuclei were stained with DAPI (blue). (B) Macrophage infiltration was identified by CD68 (green) staining. The nuclei were stained with DAPI (blue). (C) The new regenerated myofibers were identified by eMyHC (red) staining. The muscle fiber membrane was stained with laminin-a2 (green). (D) Quantification of necrotic fibers. (E) Quantification of CD68 positive area. (F) Quantification of eMyHC positive fibers. Error bars indicate ‘mean + SD’, n = 6, *p < 0.05. Scale bar = 50 µm (A,B). Scale bar = 25 µm (C).