Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Apr 27;10:7078. doi: 10.1038/s41598-020-63796-w

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Recruitment of neutrophils and nitrotyrosine formation with anti-PD-1 treatment. (A) Representative confocal microscopy images of myeloperoxidase (red) staining of neutrophils in necrotic areas in tumors from the isotype control group, and from the experimental groups treated with cisplatin or anti-PD-1 monotherapy. Nuclei were stained with TO-PRO-3 (blue). Right panels show merged images. Scale bars, 100 µm. (B) Nitrotyrosine labeling in necrotic areas from the anti-PD-1 treatment group. Representative images of nitrotyrosine (green) localization by confocal microscopy in each study group. Nuclei were stained with TO-PRO-3 (blue). Right panels show merged images. Scale bars, 100 µm. (C) Detection of myeloperoxidase and nitrotyrosine in infiltrated tumor cells of mice treated with anti-PD-1. Representative confocal microscopy images of expression and localization of myeloperoxidase (red) and nitrotyrosine (green) in tumors from the control group and from tumors treated with anti-PD-1 monotherapy. Right panel shows merged images of co-localization (yellow). Scale bars, 5 µm.